src family tyrosine kinase inhibitors pp1 Search Results


src tyrosine kinase substrate  (Biomol GmbH)
90
Biomol GmbH src tyrosine kinase substrate
Src Tyrosine Kinase Substrate, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/src+family+tyrosine+kinase+inhibitors+pp1/pmc02581794-197-0-7?v=Biomol+GmbH
Average 90 stars, based on 1 article reviews
src tyrosine kinase substrate - by Bioz Stars, 2026-07
90/100 stars
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src-family tyrosine kinase inhibitor, pp1  (AG Scientific)
90
AG Scientific src-family tyrosine kinase inhibitor, pp1
GPR30-mediated FN matrix assembly occurs independently of EGFR transactivation in SKBR3 breast cancer cells. A, SKBR3 cells were fed rat FN (rFN) (25 μg/ml) in the presence of either 17β-E2 (1 nm) or ICI 182,780 (1 μm) or no agonist (untreated, UN) for 18 h. Alternatively, cells were pretreated with the pan MMP-inhibitor, GM6001 (10 μm for 15 min), Src-like kinase inhibitor, <t>PP1</t> (50 nm for 15 min), or CRM-197 (200 ng/ml for 1 h) before receiving rFN in the presence of 17β-E2. Equivalent amounts of DOC-soluble or -insoluble protein were blotted with rFN-specific mAB IC3. The immunoblot shown is a representative of four independent experiments. Band intensities from individual experiments were measured using Scion Image software. Mean integrated optical densities (±sem) were measured for unstimulated (28.6 ± 11.5) and 17β-E2-stimulated (148.1 ± 15.7) cells. B, SKBR3 cells were pretreated with either the erbB1-specific inhibitor, tyrphostin AG1478 (1 nm) or vehicle (DMSO) for 15 min before receiving rFN in the presence of 17β-E2 (1 nm), ICI 182, 780 (1 μm), EGF (10 ng/ml), HB-EGF (10 ng/ml), or no agonist. FN matrix assembly was measured after 18 h of treatment as described above. C, SKBR3 cells treated with AG1478 or vehicle as described in panel B in the absence of exogenous rFN. Cells were then lysed and erbB1 tyrosyl phosphorylation was assessed in erbB1 immunoprecipitates that were immunoblotted with phosphotyrosine-specific 4G10 mAB. ErbB1 recovery was determined by reprobing the same filter with sheep EGFR antibodies. DMSO, Dimethylsulfoxide.
Src Family Tyrosine Kinase Inhibitor, Pp1, supplied by AG Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/src+family+tyrosine+kinase+inhibitors+pp1/pmc02703602-466-4-8?v=AG+Scientific
Average 90 stars, based on 1 article reviews
src-family tyrosine kinase inhibitor, pp1 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
src-family tyrosine kinase inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl) pyrazolo [3,4-d]pyrimidine (pp2)  (Biomol GmbH)
90
Biomol GmbH src-family tyrosine kinase inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl) pyrazolo [3,4-d]pyrimidine (pp2)
GPR30-mediated FN matrix assembly occurs independently of EGFR transactivation in SKBR3 breast cancer cells. A, SKBR3 cells were fed rat FN (rFN) (25 μg/ml) in the presence of either 17β-E2 (1 nm) or ICI 182,780 (1 μm) or no agonist (untreated, UN) for 18 h. Alternatively, cells were pretreated with the pan MMP-inhibitor, GM6001 (10 μm for 15 min), Src-like kinase inhibitor, <t>PP1</t> (50 nm for 15 min), or CRM-197 (200 ng/ml for 1 h) before receiving rFN in the presence of 17β-E2. Equivalent amounts of DOC-soluble or -insoluble protein were blotted with rFN-specific mAB IC3. The immunoblot shown is a representative of four independent experiments. Band intensities from individual experiments were measured using Scion Image software. Mean integrated optical densities (±sem) were measured for unstimulated (28.6 ± 11.5) and 17β-E2-stimulated (148.1 ± 15.7) cells. B, SKBR3 cells were pretreated with either the erbB1-specific inhibitor, tyrphostin AG1478 (1 nm) or vehicle (DMSO) for 15 min before receiving rFN in the presence of 17β-E2 (1 nm), ICI 182, 780 (1 μm), EGF (10 ng/ml), HB-EGF (10 ng/ml), or no agonist. FN matrix assembly was measured after 18 h of treatment as described above. C, SKBR3 cells treated with AG1478 or vehicle as described in panel B in the absence of exogenous rFN. Cells were then lysed and erbB1 tyrosyl phosphorylation was assessed in erbB1 immunoprecipitates that were immunoblotted with phosphotyrosine-specific 4G10 mAB. ErbB1 recovery was determined by reprobing the same filter with sheep EGFR antibodies. DMSO, Dimethylsulfoxide.
Src Family Tyrosine Kinase Inhibitor 4 Amino 5 (4 Chlorophenyl) 7 (T Butyl) Pyrazolo [3,4 D]Pyrimidine (Pp2), supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/src+family+tyrosine+kinase+inhibitors+pp1/pm19319148-57-0-8?v=Biomol+GmbH
Average 90 stars, based on 1 article reviews
src-family tyrosine kinase inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl) pyrazolo [3,4-d]pyrimidine (pp2) - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
src family tyrosine kinase inhibitor protein phosphatase 1 (pp1)  (Biomol GmbH)
90
Biomol GmbH src family tyrosine kinase inhibitor protein phosphatase 1 (pp1)
GPR30-mediated FN matrix assembly occurs independently of EGFR transactivation in SKBR3 breast cancer cells. A, SKBR3 cells were fed rat FN (rFN) (25 μg/ml) in the presence of either 17β-E2 (1 nm) or ICI 182,780 (1 μm) or no agonist (untreated, UN) for 18 h. Alternatively, cells were pretreated with the pan MMP-inhibitor, GM6001 (10 μm for 15 min), Src-like kinase inhibitor, <t>PP1</t> (50 nm for 15 min), or CRM-197 (200 ng/ml for 1 h) before receiving rFN in the presence of 17β-E2. Equivalent amounts of DOC-soluble or -insoluble protein were blotted with rFN-specific mAB IC3. The immunoblot shown is a representative of four independent experiments. Band intensities from individual experiments were measured using Scion Image software. Mean integrated optical densities (±sem) were measured for unstimulated (28.6 ± 11.5) and 17β-E2-stimulated (148.1 ± 15.7) cells. B, SKBR3 cells were pretreated with either the erbB1-specific inhibitor, tyrphostin AG1478 (1 nm) or vehicle (DMSO) for 15 min before receiving rFN in the presence of 17β-E2 (1 nm), ICI 182, 780 (1 μm), EGF (10 ng/ml), HB-EGF (10 ng/ml), or no agonist. FN matrix assembly was measured after 18 h of treatment as described above. C, SKBR3 cells treated with AG1478 or vehicle as described in panel B in the absence of exogenous rFN. Cells were then lysed and erbB1 tyrosyl phosphorylation was assessed in erbB1 immunoprecipitates that were immunoblotted with phosphotyrosine-specific 4G10 mAB. ErbB1 recovery was determined by reprobing the same filter with sheep EGFR antibodies. DMSO, Dimethylsulfoxide.
Src Family Tyrosine Kinase Inhibitor Protein Phosphatase 1 (Pp1), supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/src+family+tyrosine+kinase+inhibitors+pp1/10__1111_slash_j__1524___475x__2009__00563__x-75-16-48?v=Biomol+GmbH
Average 90 stars, based on 1 article reviews
src family tyrosine kinase inhibitor protein phosphatase 1 (pp1) - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


GPR30-mediated FN matrix assembly occurs independently of EGFR transactivation in SKBR3 breast cancer cells. A, SKBR3 cells were fed rat FN (rFN) (25 μg/ml) in the presence of either 17β-E2 (1 nm) or ICI 182,780 (1 μm) or no agonist (untreated, UN) for 18 h. Alternatively, cells were pretreated with the pan MMP-inhibitor, GM6001 (10 μm for 15 min), Src-like kinase inhibitor, PP1 (50 nm for 15 min), or CRM-197 (200 ng/ml for 1 h) before receiving rFN in the presence of 17β-E2. Equivalent amounts of DOC-soluble or -insoluble protein were blotted with rFN-specific mAB IC3. The immunoblot shown is a representative of four independent experiments. Band intensities from individual experiments were measured using Scion Image software. Mean integrated optical densities (±sem) were measured for unstimulated (28.6 ± 11.5) and 17β-E2-stimulated (148.1 ± 15.7) cells. B, SKBR3 cells were pretreated with either the erbB1-specific inhibitor, tyrphostin AG1478 (1 nm) or vehicle (DMSO) for 15 min before receiving rFN in the presence of 17β-E2 (1 nm), ICI 182, 780 (1 μm), EGF (10 ng/ml), HB-EGF (10 ng/ml), or no agonist. FN matrix assembly was measured after 18 h of treatment as described above. C, SKBR3 cells treated with AG1478 or vehicle as described in panel B in the absence of exogenous rFN. Cells were then lysed and erbB1 tyrosyl phosphorylation was assessed in erbB1 immunoprecipitates that were immunoblotted with phosphotyrosine-specific 4G10 mAB. ErbB1 recovery was determined by reprobing the same filter with sheep EGFR antibodies. DMSO, Dimethylsulfoxide.

Journal:

Article Title: Coordinate Regulation of Estrogen-Mediated Fibronectin Matrix Assembly and Epidermal Growth Factor Receptor Transactivation by the G Protein-Coupled Receptor, GPR30

doi: 10.1210/me.2008-0262

Figure Lengend Snippet: GPR30-mediated FN matrix assembly occurs independently of EGFR transactivation in SKBR3 breast cancer cells. A, SKBR3 cells were fed rat FN (rFN) (25 μg/ml) in the presence of either 17β-E2 (1 nm) or ICI 182,780 (1 μm) or no agonist (untreated, UN) for 18 h. Alternatively, cells were pretreated with the pan MMP-inhibitor, GM6001 (10 μm for 15 min), Src-like kinase inhibitor, PP1 (50 nm for 15 min), or CRM-197 (200 ng/ml for 1 h) before receiving rFN in the presence of 17β-E2. Equivalent amounts of DOC-soluble or -insoluble protein were blotted with rFN-specific mAB IC3. The immunoblot shown is a representative of four independent experiments. Band intensities from individual experiments were measured using Scion Image software. Mean integrated optical densities (±sem) were measured for unstimulated (28.6 ± 11.5) and 17β-E2-stimulated (148.1 ± 15.7) cells. B, SKBR3 cells were pretreated with either the erbB1-specific inhibitor, tyrphostin AG1478 (1 nm) or vehicle (DMSO) for 15 min before receiving rFN in the presence of 17β-E2 (1 nm), ICI 182, 780 (1 μm), EGF (10 ng/ml), HB-EGF (10 ng/ml), or no agonist. FN matrix assembly was measured after 18 h of treatment as described above. C, SKBR3 cells treated with AG1478 or vehicle as described in panel B in the absence of exogenous rFN. Cells were then lysed and erbB1 tyrosyl phosphorylation was assessed in erbB1 immunoprecipitates that were immunoblotted with phosphotyrosine-specific 4G10 mAB. ErbB1 recovery was determined by reprobing the same filter with sheep EGFR antibodies. DMSO, Dimethylsulfoxide.

Article Snippet: Src-family tyrosine kinase inhibitor, PP1, was purchased from AG Scientific Inc. (San Diego, CA).

Techniques: Western Blot, Software, Phospho-proteomics

GPR30 signaling promotes the formation of Src-dependent, Shc-integrin α5β1 protein complexes. Triton X-100 extracts prepared from MDA-MB-231 cells stably expressing vector or GPR30 were left untreated (UN) or stimulated with 17β-E2, EGF, or pretreated with the Src family kinase inhibitor, PP1 (50 nm; 15 min) before stimulation with 17β-E2. Integrin α5β1 or α2β1 was immunoprecipitated with rabbit antibodies specific for the integrin α5 or α2 subunit cytoplasmic tails, respectively. Associated Shc proteins were detected with Shc mAB, PG797. Recovery of integrin α5β1 or α2β1 was determined by blotting with integrin α5 or α2 subunit protein antibodies. Relative positions of endogenous p66, p52, and p46 Shc are designated.

Journal:

Article Title: Coordinate Regulation of Estrogen-Mediated Fibronectin Matrix Assembly and Epidermal Growth Factor Receptor Transactivation by the G Protein-Coupled Receptor, GPR30

doi: 10.1210/me.2008-0262

Figure Lengend Snippet: GPR30 signaling promotes the formation of Src-dependent, Shc-integrin α5β1 protein complexes. Triton X-100 extracts prepared from MDA-MB-231 cells stably expressing vector or GPR30 were left untreated (UN) or stimulated with 17β-E2, EGF, or pretreated with the Src family kinase inhibitor, PP1 (50 nm; 15 min) before stimulation with 17β-E2. Integrin α5β1 or α2β1 was immunoprecipitated with rabbit antibodies specific for the integrin α5 or α2 subunit cytoplasmic tails, respectively. Associated Shc proteins were detected with Shc mAB, PG797. Recovery of integrin α5β1 or α2β1 was determined by blotting with integrin α5 or α2 subunit protein antibodies. Relative positions of endogenous p66, p52, and p46 Shc are designated.

Article Snippet: Src-family tyrosine kinase inhibitor, PP1, was purchased from AG Scientific Inc. (San Diego, CA).

Techniques: Stable Transfection, Expressing, Plasmid Preparation, Immunoprecipitation